With this assay, the presence and location of proteins can be demonstrated in tissue sections.
The aim of these immunohistochemical procedures is to investigate
In vitro, the following tissues can be investigated:
The evaluation method can be qualitative, semi-quantitative and quantitative (morphometrical analysis, cell counting).
Establishment of staining procedure (selection of antibody – primary and detection system, positive and negative control tissues, optimization of fixation, antibody concentrations, incubation time, blocking method)
Validation of the staining procedure (if necessary) by testing the following parameters:
Depending on the type of evaluation: