Immunohistochemistry of immune tissues

Aim:

With this assay, the presence and location of proteins can be demonstrated in tissue sections.

The aim of these immunohistochemical procedures is to investigate

the localization and tissue distribution of target molecules,
the changes in the expression level/concentration of target molecules in the tissue by fluorescent microscopy (3 channels).

Test system:

In vitro, the following tissues can be investigated:

Formalin fixed paraffin-embedded (FFPE) tissues
Frozen sections
Human, NHP, other animal tissues (healthy or specific disease panel, full tissue array from commercial source)

Design:

The evaluation method can be qualitative, semi-quantitative and quantitative (morphometrical analysis, cell counting).

Establishment of staining procedure (selection of antibody – primary and detection system, positive and negative control tissues, optimization of fixation, antibody concentrations, incubation time, blocking method)

Validation of the staining procedure (if necessary) by testing the following parameters:

Specificity
Sensitivity
Repeatability
Dose-dependence

Outcome:

Depending on the type of evaluation:

Qualitative – negative or positive
Semi-quantitative – scoring
Quantitative – number of cells, area of positivity per visual field