One of the most important tools to follow the changes in the immune function/immune status due to the influence of a test item is the determination of the number/ratio of the different immune cells applying specific labeling of cell phenotype markers of the immune cells. This can be part of Part of a toxicity study or clinical trial.
Usually two species: one rodent and one non-rodent.
Establishment of the staining procedure and the evaluation of the samples with flow cytometer.
Validation of the staining procedure testing the following parameters:
Ratio of the following cell types
In case the number of total lymphocytes is determined, the absolute number of subtypes can be also calculated.